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Tetanus Toxoid (TTx)-Induced Delayed Type Hypersensitivity (DTH) Model

DTH is a classic model of Type IV hypersensitivity reactions driven by an antigen specific T cell response resulting in inflammation and tissue injury. In preclinical studies, DTH models are widely used for the evaluation of novel immunosuppressive and immunomodulatory agents, as well as in vaccine development.

Case Study:TTx-Induced DTH in Cynomolgus Monkey

Animal: Cynomolgus monkeys, 2.5 - 3.3 kg 

Material: Tetanus Toxoid (TTx) Vaccine

DTH Response in Cynomolgus Monkey


DTH expressed as skin induration on the challenged spots and treatment with topical Dex or systemic cmpd-X attenuated this response.    

Skin Erythema, Edema, Indurations



Skin Histopathology Evaluation


IHC: Immune Cell Infiltrate Determination: Examination


Monocytes, B &T Cells, and NK Cells in Peripheral Blood


Similar to Dexamethasone, the cmpd-X treatment group showed a decrease in CD14+ monocyte and classical monocyte (CD14+CD16-). However, levels of activated monocyte (CD14+CD16+) and other major immune cell populations, e.g. B cells, T cells, NK cells, were not affected as compared to vehicle group.    

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