Allergy & Hypersensitivity

Prime models for allergy and hypersensitivity studies.

According to a 2020 study done by the U.S. Department of Health and Human Services, food allergies alone in the United States lead to a total 30,000 Emergency Room visits per year, costing roughly $25 billion. 

To this day, allergies still have no cures: hypersensitivity management is mainly preventive, with mitigation of symptoms when they occur. Whether you are developing a vaccine or evaluating novel immunomodulatory and immunosuppressive agents, choosing the right CRO to provide you with all the data you need is crucial. This is why we always go the extra mile at PharmaLegacy; Our highly trained-staff are available 24/7 to answer all your questions. Our allergy and hypersensitivity models are state of the art. Keep scrolling and we’ll show you some.

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Allergy & Hypersensitivity

MODELS / SERVICES

PharmaLegacy Research Tools

Rodent Model Characteristics:

·       Peak response at 24-48h, rare erythema and different numbers of lymphocytes/monocytes across different models;

·       High throughput and requirement for smaller quantity of compound;

·       Low variability between subjects using inbred animals;

·       Higher concentration of antigen/hapten for induction in certain strains.

Measurement of Inflammatory Responses and Molecular Pharmacology:

·       ear/paw swelling

·       myeloperoxidase assay (neutrophils and granulocytes)

·       cytokine production: TNFα, interferon γ, IL-1β, IL-4, IL-6, Cox-2, (ELISA or Q-PCR)

Histopathology:

·       H&E staining: induration and infiltrations.

·       Immunohistochemistry: specific T cell response and cell proliferation.

Pharmalegacy has three types models of Oxazolone -induced DTH:

·        Oxazolone-induced DTH in ICR mice

·        Oxazolone-induced DTH in BALB/c mice

Case Study - Oxazolone Induced DTH in ICR Mice:

Aim: To examine the effect of dexamethasone on oxazolone-induced DTH.

Animal: ICR mice, female, 20-22 g

Reagent: Oxazolone; Acetone and Olive oil; Dexamethasone (Dex).

Effect of Dexamethasone on Ear Thickness

Effect of Dexamethasone on IL-1, IL-6, and TNF-a mRNA in Ears

Ear Pathology (H & E staining, x40)

Oxazolone-induced DTH in BALB/c Mice

Aim: To validate the oxazolone-induced DTH in BALB/C mice model, and to study the effect of Dexamethasone on oxazolone-induced DTH.

Animal: BALB/c mice, female, 17-19 g

Reagent: Oxazolone,  Reference drug: Dexamethasone.

Dosing Schedule and Result

PharmaLegacy Research Tools

Rodent Model Characteristics:

·       Peak response at 24-48h, rare erythema and different numbers of lymphocytes/monocytes across different models;

·       High throughput and requirement for smaller quantity of compound;

·       Low variability between subjects using inbred animals;

·       Higher concentration of antigen/hapten for induction in certain strains.

Measurement of Inflammatory Responses and Molecular Pharmacology:

·       Ear/paw swelling;

·       Myeloperoxidase assay (neutrophils and granulocytes);

·       Cytokine production: TNFα, interferon γ, IL-1β, IL-4, IL-6, Cox-2, (ELISA or Q-PCR).

Histopathology:

·         H&E staining: induration and infiltrations

·         Immunohistochemistry: specific T cell response and cell proliferation

Pharmalegacy has three types models of DNFB-induced DTH:

·       DNFB-induced DTH in ICR mice (preventive or therapeutic);

·       DNFB-induced DTH in wistar rats;

·       DNFB-induced DTH in BALB/c mice.

DNFB-Induced DTH in Wistar Rats

Aim: To examine the effect of dexamethasone on DNFB-induced DTH in Wistar rats

Animal: Wistar rats, male,180-200 g

Reagent: DNFB and Dexamethasone, Acetone, Olive oil, Dexamethasone tablet

Effect of Dex on Ear Thickness and MPO activity

Effect of Dex on IL-1b, IL-6, and TNF-a mRNA in Ears

Ear Pathology (H & E Staining)

Case Study:TTx-Induced DTH in Cynomolgus Monkey

Animal: Cynomolgus monkeys, 2.5 – 3.3 kg

Material: Tetanus Toxoid (TTx) Vaccine

DTH Response in Cynomolgus Monkey

DTH expressed as skin induration on the challenged spots and treatment with topical Dex or systemic cmpd-X attenuated this response.

Skin Erythema, Edema, Indurations

Skin Histopathology Evaluation

IHC: Immune Cell Infiltrate Determination: Examination

Monocytes, B &T Cells, and NK Cells in Peripheral Blood

Similar to Dexamethasone, the cmpd-X treatment group showed a decrease in CD14+ monocyte and classical monocyte (CD14+CD16-). However, levels of activated monocyte (CD14+CD16+) and other major immune cell populations, e.g. B cells, T cells, NK cells, were not affected as compared to vehicle group.

Passive Cutaneous Anaphylaxis in Male BALB/c Mice

Aim: To validate passive cutaneous anaphylaxis model in male BALB/c mice.

Animal: BALB/c mice

Reagent: Anti-DNP IgE, Albumin dinitrophenyl (DNP-BSA), Evan’s Blue, Periactin

Dosing Schedule and Result

Passive Cutaneous Anaphylaxis in Male Wistar Rats

Aim: To validate passive cutaneous anaphylaxis model in male Wistar rats.

Animal: Wistar rats

Reagent: Anti-DNP IgE, Albumin dinitrophenyl (DNP-BSA), Evan’s Blue, Periactin

Dosing Schedule and Result

The Capabilities to Succeed:

  • Operations and IT structured for maximum protection of clients’ data and IP
  • Capacity to house over 20,000 rodents and large animals
  • On-site capacity for up to 350 non-human primates, with off-site capacity for up to 30,000 Cynomolgus and Rhesus monkeys
  • 24/7 access to PharmaLegacy representatives

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